Journal: Cells

Article Title: Involvement of Cyclooxygenase-2 in Establishing an Immunosuppressive Microenvironment in Tumorspheres Derived from TMZ-Resistant Glioblastoma Cell Lines and Primary Cultures

doi: 10.3390/cells13030258

Figure Lengend Snippet: List of primary antibodies used in the present study.

Article Snippet: rabbit monoclonal anti-osteopontin , 1:1000 , Boster Biological Technology, Pleasanton, CA, USA.

Techniques:

Journal: Arthritis Research & Therapy

Article Title: Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis-induced inflammation, synovial hyperplasia, and cartilage destruction in rats

doi: 10.1186/s13075-018-1547-6

Figure Lengend Snippet: Collagen-induced arthritis (CIA) enhanced osteopontin (OPN) and cartilage oligomeric matrix protein (COMP) expression in paw joint cartilage, while the Bi-Qi capsule or methotrexate (MTX) treatment reduced this effect. Representative immunohistochemistry images of paw joint synovium with OPN and COMP immunostaining

Article Snippet: After blocking of non-specific binding sites with 10% normal goat serum for 1 h, the sections were incubated overnight at 4 °C with mouse-anti-COMP antibody (Boiss antibodies company, Beijing, China) in PBS or mouse anti-OPN antibody (Boster Bioengineering Co.Ltd., Wuhan, China) in PBS.

Techniques: Expressing, Immunohistochemistry, Immunostaining

Journal: Arthritis Research & Therapy

Article Title: Traditional Chinese medicine formula Bi-Qi capsule alleviates rheumatoid arthritis-induced inflammation, synovial hyperplasia, and cartilage destruction in rats

doi: 10.1186/s13075-018-1547-6

Figure Lengend Snippet: Bi-Qi capsule (BQ) or methotrexate (MTX) reduced collagen-induced arthritis (CIA)-induced osteopontin (OPN) and cartilage oligomeric matrix protein (COMP) upregulation in serum, and mRNA and protein levels in paw joint tissue. a, b OPN and COMP levels in serum. c, d OPN and COMP mRNA expression in paw joint cartilage. e, f OPN and COMP protein quantification from immunohistochemistry images of paw joint synovium. Data are presented as mean ± SD from eight rats in each group. Significant effect of treatment compared to control: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 . Significant effect of treatment compared to arthritic group: #### P < 0.0001 . Significant effect of treatment compared to high-dose BQ (BQ-HD): $$ P < 0.01, $$$$ P < 0.0001 . Significant effect of treatment compared to MTX-group: & P < 0.05, &&&& P < 0.0001. BQ-MD, moderate-dose BQ

Article Snippet: After blocking of non-specific binding sites with 10% normal goat serum for 1 h, the sections were incubated overnight at 4 °C with mouse-anti-COMP antibody (Boiss antibodies company, Beijing, China) in PBS or mouse anti-OPN antibody (Boster Bioengineering Co.Ltd., Wuhan, China) in PBS.

Techniques: Expressing, Immunohistochemistry, Control

Journal: International Journal of Molecular Sciences

Article Title: LPS Disrupts Endometrial Receptivity by Inhibiting STAT1 Phosphorylation in Sheep

doi: 10.3390/ijms252413673

Figure Lengend Snippet: Effect of LPS on endometrial receptivity genes in sheep. ( A ) The pro-conceptus elongation gene ISG15 , RSAD2 ( B ), and CXCL10 ( C ) on d12, d16, and d20 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. ( D ) The adhesion molecules ITGB1 , ITGB3 ( E ), ITGB5 ( F ), SPP1 ( G ), and MUC1 ( H ) on d12 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. ( I ) The endometrial receptivity markers HOXA10 , HOXA11 ( J ), and LIF ( K ) on d12 of pregnancy in endometrial tissue were measured by real-time quantitative PCR. All data are presented as the mean ± SEM, n ≥ 3; * p < 0.05; ** p < 0.01.

Article Snippet: Subsequently, the permeabilized cells were blocked with 2% BSA for 1 h and incubated with primary antibodies against SPP1 (1:200; WL02378; Wanleibio Co., Ltd., Shenyang, China) and cytokeratin 18 (1:200; AF0191; Affinity Biosciences, Cincinnati, OH, USA) at 4 °C overnight.

Techniques: Real-time Polymerase Chain Reaction

Journal: International Journal of Molecular Sciences

Article Title: LPS Disrupts Endometrial Receptivity by Inhibiting STAT1 Phosphorylation in Sheep

doi: 10.3390/ijms252413673

Figure Lengend Snippet: Effect of LPS or fludarabine treatment on the expression of endometrial receptivity-related genes under hormone treatment. ( A ) The protein level of p-STAT1 and T-STAT1 in sEECs. ( B ) The secretion of PGE2 and PGF2α in sEECs. ( C – E ) The pro-conceptus elongation genes ISG15 , RSAD2 , CXCL10 , adhesion molecules ITGB1/3/5 , MUC1 , SPP1 , and receptivity markers HOXA10 , HOXA11 , LIF mRNA expression levels in sEECs. GAPDH (sheep) was used as the reference gene in all samples. ( F ) Confocal microscope images of SPP1 expression in four treatment groups. Red: Cy3-labeled SPP1 protein; blue, DAPI-labeled nuclei; scale bar: 20 µm. All data are presented as the mean ± SEM, n ≥ 3; * p < 0.05; ** p < 0.01.

Article Snippet: Subsequently, the permeabilized cells were blocked with 2% BSA for 1 h and incubated with primary antibodies against SPP1 (1:200; WL02378; Wanleibio Co., Ltd., Shenyang, China) and cytokeratin 18 (1:200; AF0191; Affinity Biosciences, Cincinnati, OH, USA) at 4 °C overnight.

Techniques: Expressing, Microscopy, Labeling

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: ( a ): Baseline clinical and pathological characteristics of bladder cancer patients. ( b ): Median and IQR of patients’ age and follow-up duration. ( c ): Expression intensity of SPP1.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Staining

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Expression pattern of SPP1 in pan-cancer. ( A ) The expression of the SPP1 mRNA level in multiple TCGA cancers and matching normal tissues. p < 0.001, except Thym cancer ( p = 0.7) and KICH cancer ( p = 0.53). ( B ) Increased mRNA expression level of SPP1 in bladder cancer. ( C ) Promoter methylation status of SPP1 in bladder cancer and matching normal tissues. All data were analyzed using the UALCAN web tool.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Methylation

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Molecular alterations of SPP1 in cancers. ( A ) High amplifications/mutations of the SPP1 gene in bladder cancer compared other cancer types. ( B ) Positions and mutation frequency in SPP1 in bladder cancer.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Mutagenesis

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Cytoplasmic expression of SPP1 in bladder carcinoma. Immunohistochemical staining of the bladder cancer tissue microarray using an SPP1 antibody. Figures showing: no expression ( A , B ), weak ( C , D ), moderate ( E , F ) and strong expression ( G , H ) of SPP1. Images were taken using 10× and 40× magnification objectives (scale bar equals 1 mm).

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Immunohistochemical staining, Staining, Microarray

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Nuclear SPP1 expression in bladder carcinoma. Immunohistochemical staining of the bladder cancer tissue microarray using an SPP1 antibody. Figures showing: no expression ( C , D ), and strong expression of SPP1 ( A , B ). Images were taken with 10× and 40× magnification objectives (scale bar equals 1 mm).

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Immunohistochemical staining, Staining, Microarray

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Correlation between cytoplasmic SPP1 expression and patients’ clinicopathological characteristics.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: SPP1 expression and patients’ survival. Kaplan–Meier survival curve for bladder cancer patients expressing cytoplasmic ( A ) and nuclear ( B ) patterns of SPP1 (low expression vs. high expression). Low SPP1 immunostaining is associated with poor overall survival (log-rank p = 0.022).

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Immunostaining

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Enrichment analysis of SPP1 in bladder cancer. ( A ) Identification of SPP1-interacting genes. Protein–protein interaction map and hub genes of SPP1. The size of the hub is proportional to the expression level. ( B ) Identification of the SPP1 co-expression network. The figure was generated using the online cBioPortal database. ( C ) KEGG functional enrichment analysis of SPP1. The figure was generated using the online cBioPortal database.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing, Generated, Functional Assay

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Correlation between immune cells and SPP1 expression. TIMER analysis of the correlation between SPP1 expression and immune cells’ infiltration. Purity-adjusted Spearman’s rho across various cell types by different algorithms.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing

Journal: Cancers

Article Title: Identification of SPP1 as a Prognostic Biomarker and Immune Cells Modulator in Urothelial Bladder Cancer: A Bioinformatics Analysis

doi: 10.3390/cancers15235704

Figure Lengend Snippet: Relationship between SPP1 expression and immune checkpoint genes in bladder cancer. ( A ) Correlation analysis between SPP1 expression and immune checkpoint genes. ( B ) The expression of immune checkpoint genes in relation to SPP1 expression. Data were analyzed using the cBioPortal cancer genomics website on TCGA data. The p -value significance codes: *** ≤0.001, ** ≤0.01, * ≤0.05.

Article Snippet: The slides were then incubated with the primary polyclonal human anti-SPP1 antibody (Spring Bioscience, Pleasanton, CA, USA. cat#E3284) at a dilution of 1:100 for 32 min.

Techniques: Expressing

Journal: Journal of Cardiovascular Development and Disease

Article Title: A Single-Cell Atlas of the Atherosclerotic Plaque in the Femoral Artery and the Heterogeneity in Macrophage Subtypes between Carotid and Femoral Atherosclerosis

doi: 10.3390/jcdd9120465

Figure Lengend Snippet: Validation of the difference in immune-cell count through immunofluorescence staining. cDCs were labeled by CLEC10A. Resident macrophages were labeled by F13A1. Regulatory T cells were labeled by LGALS3. Monocytes were labeled by S100A8. Foamy macrophages were labeled by SPP1. Mast cells were labeled by TPSAB1.

Article Snippet: After blocking with 3% bovine serum albumin (BSA), the sections were incubated with CLEC10A antibody (Servicebio, GB114760, 1:500), F13A1 antibody (Servicebio, GB113293, 1:2400), LGALS3 antibody (Servicebio, GB111145, 1:1000), S100A8 antibody (Servicebio, GB11421, 1:1500), SPP1 antibody (Servicebio, GB112328, 1:500), and TPSAB1 (Servicebio, GB12110, 1:500) overnight at 4 °C.

Techniques: Biomarker Discovery, Cell Counting, Immunofluorescence, Staining, Labeling